5VIM
Crystal structure of the Zika virus NS5 methyltransferase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-09 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.157, 109.735, 55.387 |
Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
Resolution | 30.514 - 2.100 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kqr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.712 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.510 |
High resolution limit [Å] | 2.100 |
Number of reflections | 27143 |
<I/σ(I)> | 4.25 |
Completeness [%] | 97.1 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 0.2M Lithium Sulfate, 0.1M Cacodylate, 30% PEG 400 |