5VHG

Crystal structure of pentad mutant GAPR-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	62.307, 84.665, 51.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.708 - 1.270
R-factor	0.1512
Rwork	0.150
R-free	0.17950
RMSD bond length	0.007
RMSD bond angle	0.879
Data reduction software	AUTOMAR
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.708
High resolution limit [Å]	1.270
Number of reflections	35987
<I/σ(I)>	11
Completeness [%]	93.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Bis-Tris pH 5.5, PEG 3350, Li2SO4.