5VH0
RHCC in complex with pyrene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-09-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 35.023, 76.995, 35.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.831 - 2.055 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fe6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.408 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.250 |
| High resolution limit [Å] | 2.055 |
| Rmerge | 0.157 |
| Rpim | 0.096 |
| Number of reflections | 11575 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 99.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350,0.1M Bis-Tris pH 5.5, 200mM NaCl |
