5VGW
RNA hairpin structure containing tetraloop/receptor motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2015-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 71.055, 71.055, 69.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.150 - 2.420 |
| R-factor | 0.20764 |
| Rwork | 0.207 |
| R-free | 0.21293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.755 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.639 |
| Number of reflections | 4799 | 508 |
| <I/σ(I)> | 15 | 2.33 |
| Completeness [%] | 96.1 | 98.8 |
| Redundancy | 3.7 | 3.1 |
| CC(1/2) | 0.914 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50 mM MgCl2, 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 7.0, 25% w/v Polyethylene glycol 3,350 |
