5VGT
X-ray structure of bacteriophage Sf6 tail adaptor protein gp7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.297, 101.297, 76.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.945 - 1.776 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23350 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.550 | 3.340 | 1.810 |
High resolution limit [Å] | 1.776 | 3.120 | 1.780 |
Rmerge | 0.057 | 0.867 | 0.679 |
Rmeas | 0.062 | 0.941 | 0.790 |
Rpim | 0.024 | 0.361 | 0.395 |
Number of reflections | 38487 | 1332 | 1936 |
<I/σ(I)> | 17.5 | ||
Completeness [%] | 98.9 | 99.8 | 89.2 |
Redundancy | 6.1 | 6.7 | 3.6 |
CC(1/2) | 0.999 | 0.770 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 8000, MES 6.0, calcium acetate |