5VGL
Crystal structure of lachrymatory factor synthase from Allium cepa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-08-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.072, 40.127, 98.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.162 - 1.400 |
R-factor | 0.1537 |
Rwork | 0.152 |
R-free | 0.18870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f08 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.130 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 34456 | 9045 |
<I/σ(I)> | 8.4 | 1.8 |
Completeness [%] | 97.8 | 94.5 |
Redundancy | 4.1 | 4 |
CC(1/2) | 0.995 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1 M sodium acetate, pH 4.5 containing PEG 3350 at concentrations between 25%-32% (w/v). |