5VGE
Crystal structure of HLA-C*07:02 in complex with RYR peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.670, 99.670, 77.914 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.160 - 2.600 |
| R-factor | 0.16629 |
| Rwork | 0.163 |
| R-free | 0.22082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nt6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.160 |
| High resolution limit [Å] | 2.600 |
| Rpim | 0.050 |
| Number of reflections | 13655 |
| <I/σ(I)> | 14.8 |
| Completeness [%] | 100.0 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M HEPES pH 8.5 2 mM ZnSO4 28 % jeffamine ED-2001 (Hampton) |
