5VG7
Crystal Structure of the R503Q missense variant of human PGM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-02-02 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 172.131, 172.131, 99.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.857 - 1.950 |
R-factor | 0.171520290168 |
Rwork | 0.169 |
R-free | 0.21294 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5epc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.950 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.140 | 1.902 |
Rpim | 0.038 | 0.526 |
Number of reflections | 108994 | 5314 |
<I/σ(I)> | 15.1 | 1.5 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 14.3 | 14 |
CC(1/2) | 0.999 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Tris/HCl pH:7.5, 1.8 M Ammonium Sulfate, 16mg/mL |