5VG2
Intradiol ring-cleavage Dioxygenase from Tetranychus urticae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 60.494, 43.067, 165.708 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.460 |
| R-factor | 0.19314 |
| Rwork | 0.191 |
| R-free | 0.23097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ilt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.666 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.077 | 0.483 |
| Rmeas | 0.116 | 0.665 |
| Rpim | 0.064 | 0.371 |
| Number of reflections | 30081 | 1539 |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 95.5 | 97.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 7.5, 15% w/v PEG6000 |
