5VFZ

Integrase from mycobacterium phage Brujita

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	133.213, 50.050, 60.679
Unit cell angles	90.00, 115.34, 90.00

Refinement procedure

Resolution	21.699 - 1.847
R-factor	0.1641
Rwork	0.164
R-free	0.18180
RMSD bond length	0.006
RMSD bond angle	0.657
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2219: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.847
Rmerge	0.080
Rpim	0.024
Number of reflections	31131
<I/σ(I)>	40.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		273	100 mM Tris pH 7.5 5% PEG-3350