5VFY
Structure of an accessory protein of the pCW3 relaxosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953700 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.560, 120.590, 52.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.210 - 2.490 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.23300 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.11) |
Phasing software | SHELXDE |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.440 | 48.440 | 2.590 |
High resolution limit [Å] | 2.490 | 8.970 | 2.490 |
Rmerge | 0.214 | 0.091 | 0.869 |
Rmeas | 0.232 | 0.101 | 0.951 |
Rpim | 0.088 | 0.043 | 0.373 |
Number of reflections | 18698 | ||
<I/σ(I)> | 10.8 | ||
Completeness [%] | 99.5 | 93.6 | 97.2 |
Redundancy | 6.3 | 5.3 | 5.9 |
CC(1/2) | 0.984 | 0.986 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1 M Na Citrate, 0.1 M Na Cacodylate, 0.1M Phenol |