5VFE
Synaptotagmin 1 C2A domain, lead-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-12-05 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54178 |
Spacegroup name | P 32 |
Unit cell lengths | 55.040, 55.040, 77.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.988 - 1.380 |
R-factor | 0.1457 |
Rwork | 0.144 |
R-free | 0.17820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f00 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.550 | 1.450 |
High resolution limit [Å] | 1.380 | 1.380 |
Rpim | 0.022 | 0.317 |
Number of reflections | 53772 | 7848 |
<I/σ(I)> | 18 | 2.2 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 8.2 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M lithium sulfate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |