5VFE

Synaptotagmin 1 C2A domain, lead-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-12-05
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54178
Spacegroup name	P 32
Unit cell lengths	55.040, 55.040, 77.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.988 - 1.380
R-factor	0.1457
Rwork	0.144
R-free	0.17820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f00
RMSD bond length	0.007
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	1.450
High resolution limit [Å]	1.380	1.380
Rpim	0.022	0.317
Number of reflections	53772	7848
<I/σ(I)>	18	2.2
Completeness [%]	100.0	99.8
Redundancy	8.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M lithium sulfate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350