5VFA
RitR Mutant - C128D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.836, 59.708, 52.509 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 27.519 - 1.452 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.18370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u8k |
RMSD bond length | 0.008 |
RMSD bond angle | 0.954 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2450: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.046 | 0.498 |
Number of reflections | 76689 | 3384 |
<I/σ(I)> | 28.6 | 2.9 |
Completeness [%] | 99.0 | 89.1 |
Redundancy | 5.2 | 4.3 |
CC(1/2) | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1 M HEPES pH 7.5, 22.5% Jeffamine ED-2001 pH 7.0 |