5VF4

Thermus aquaticus variable protein (TaqVP) from diversity-generating retroelements (DGR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	155.039, 155.039, 202.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.814 - 2.810
R-factor	0.1793
Rwork	0.178
R-free	0.22870
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.224
Data reduction software	MOSFLM
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	134.270
High resolution limit [Å]	2.810
Number of reflections	132418
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% (w/v) 2-methyl-2,4-pentanediol, 20 mm CaCl2, 100 mM Sodium Acetate (pH 4.6)