5VEP

MOUSE KYNURENINE AMINOTRANSFERASE III, RE-REFINEMENT OF THE PDB STRUCTURE 3E2F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 43 21 2
Unit cell lengths	91.820, 91.820, 233.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.590
R-factor	0.1942
Rwork	0.191
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.769
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	29.210
High resolution limit [Å]	2.590
Rmerge	0.150
Number of reflections	29301
<I/σ(I)>	10.4
Completeness [%]	91.1
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	21% PEG 400, 150 MM CACL2, 10%, GLYCEROL, 100 MM HEPES, PH 7.5