5VE9
Structure of hACF7 EF1-EF2-GAR domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.28295 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 92.984, 92.984, 90.446 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.349 - 2.795 |
R-factor | 0.258 |
Rwork | 0.256 |
R-free | 0.27880 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.750 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.130 | 0.068 | 1.156 |
Rmeas | 0.134 | 0.070 | 1.188 |
Rpim | 0.029 | 0.016 | 0.267 |
Total number of observations | 244650 | ||
Number of reflections | 11525 | ||
<I/σ(I)> | 6.9 | ||
Completeness [%] | 100.0 | 99.8 | 99.9 |
Redundancy | 21.2 | 20.1 | 18.7 |
CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | potassium chloride, PEG3350 |