5VDE
Crystal Structure of Cu(I)-loaded yeast Atx1: Crystal Form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.976, 80.054, 50.763 |
| Unit cell angles | 90.00, 99.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.16593 |
| Rwork | 0.164 |
| R-free | 0.20589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cc8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.118 | |
| Rpim | 0.057 | 0.499 |
| Number of reflections | 34104 | 2129 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 97.3 | 89 |
| Redundancy | 4.9 | 4.7 |
| CC(1/2) | 0.996 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.1 M HEPES (pH 7.3), 24% (W/v) PEG3350 |
