Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.51201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.685, 74.852, 184.103 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.740 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f5v 3rvt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.139 | 0.684 |
| Number of reflections | 36141 | |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 MM NA ACETATE, 8% W/V PEG 4000, 15% MPD, PH 4.5 |
