5VCF
RNA hairpin structure containing tetraloop/receptor motif, complexed with 2-MeImpG analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-09-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 71.073, 71.073, 71.274 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.580 - 2.800 |
R-factor | 0.16517 |
Rwork | 0.163 |
R-free | 0.21335 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.890 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.072 | 0.512 |
Number of reflections | 3296 | 316 |
<I/σ(I)> | 0.98 | 1.53 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 50 mM MgCl2, 200 mM Lithium sulfate monohydrate, 100 mM BIS-TRIS pH 7.0, 25% w/v Polyethylene glycol 3,350 |