5VCA
VCP like ATPase from T. acidophilum (VAT)-Substrate bound conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.8 |
| Number of Particle | 171381 |
| Software | RELION,cryoSPARC,PHENIX,CTFFIND,UCSF Chimera,NAMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
