5VC9
Zinc finger of human CXXC4 in complex with CpG DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 29.983, 40.290, 57.760 |
Unit cell angles | 70.98, 86.43, 67.83 |
Refinement procedure
Resolution | 27.200 - 2.100 |
R-factor | 0.2132 |
Rwork | 0.211 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unpublished model of related protein-DNA complex |
RMSD bond length | 0.017 |
RMSD bond angle | 1.554 |
Data reduction software | HKL-3000 |
Data scaling software | Aimless (0.5.31) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.240 | 27.240 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.061 | 0.050 | 0.597 |
Rmeas | 0.081 | 0.066 | 0.820 |
Rpim | 0.054 | 0.042 | 0.559 |
Total number of observations | 28017 | 346 | 1874 |
Number of reflections | 13389 | ||
<I/σ(I)> | 10.1 | 32 | 1.3 |
Completeness [%] | 97.2 | 88.2 | 92.6 |
Redundancy | 2.1 | 2.1 | 1.8 |
CC(1/2) | 0.995 | 0.992 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 30% PEG-1500, 5% MPD, 0.2 M sodium chloride, 0.1 M HEPES |