5VBX
Crystal structure of holo-[acyl-carrier-protein] synthase (AcpS) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.376, 69.522, 59.894 |
| Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
| Resolution | 29.598 - 2.050 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f7t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.598 | |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.146 | |
| Number of reflections | 24465 | 2704 |
| <I/σ(I)> | 10.24 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.2 | 3.9 |
| CC(1/2) | 0.996 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8-2.2 M Sodium Formate 1.8-2.2 M Sodium Nitrate 100 mM HEPES pH 7.0 |
