5VBI
Crystal Structure of the R515W missense variant of human PGM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-11-03 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 172.330, 172.330, 99.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.496 - 1.750 |
| R-factor | 0.166463873181 |
| Rwork | 0.165 |
| R-free | 0.19087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5epc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.930 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.093 | 1.580 |
| Rpim | 0.025 | 0.561 |
| Number of reflections | 150294 | 6946 |
| <I/σ(I)> | 20.1 | 1.1 |
| Completeness [%] | 99.7 | 93.6 |
| Redundancy | 13.6 | 8.5 |
| CC(1/2) | 0.999 | 0.595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES 7.5, 0.1M Sodium Chloride, 1.5-1.7M Ammonium Sulfate |
