5VBC
Crystal structure of ATXR5 in complex with histone H3.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.170, 87.310, 74.510 |
Unit cell angles | 90.00, 127.78, 90.00 |
Refinement procedure
Resolution | 26.404 - 2.100 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o30 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.997 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 29410 |
<I/σ(I)> | 12.1 |
Completeness [%] | 98.3 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50% polypropylene glycol 400, 100mM Na-Hepes pH 6.0 and 5% DMSO |