5VBC

Crystal structure of ATXR5 in complex with histone H3.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	101.170, 87.310, 74.510
Unit cell angles	90.00, 127.78, 90.00

Refinement procedure

Resolution	26.404 - 2.100
R-factor	0.1981
Rwork	0.196
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o30
RMSD bond length	0.008
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	27.000
High resolution limit [Å]	2.100
Number of reflections	29410
<I/σ(I)>	12.1
Completeness [%]	98.3
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	50% polypropylene glycol 400, 100mM Na-Hepes pH 6.0 and 5% DMSO