5VAZ

Crystal structure of a DNA primase domain from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 32 2 1
Unit cell lengths	142.300, 142.300, 106.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.866 - 2.400
R-factor	0.1879
Rwork	0.187
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dd9
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.866	48.866	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.060	0.028	0.534
Rmeas	0.065	0.031	0.573
Number of reflections	48947	602	3607
<I/σ(I)>	21.11	45.96	3.68
Completeness [%]	100.0	97.4	100
Redundancy	7.486	6.281	7.6
CC(1/2)	0.999	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	PsaeA.00153.a.B7.PS38108 at 18.8 mg/mL against Top96 screen condition A4: 0.1 M Tris pH 8.5, 2 M ammonium sulfate, cryo-protected with 20% EG, crystal tracking ID 28610a4