5VAS
Pekin duck egg lysozyme isoform III (DEL-III), orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.005, 58.350, 107.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.780 - 1.400 |
R-factor | 0.1451 |
Rwork | 0.144 |
R-free | 0.16930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iee |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.780 | 27.780 | 1.420 |
High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
Rmerge | 0.091 | 0.034 | 0.912 |
Rmeas | 0.094 | 0.035 | 0.946 |
Rpim | 0.024 | 0.010 | 0.249 |
Total number of observations | 803682 | ||
Number of reflections | 55408 | ||
<I/σ(I)> | 17.8 | ||
Completeness [%] | 100.0 | 97.6 | 100 |
Redundancy | 14.5 | 12.1 | 14.1 |
CC(1/2) | 0.999 | 0.999 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.4 M ammonium phosphate monobasic, 100 mM Tris, pH 8.5 |