5VAP

Crystal structure of eVP30 C-terminus and eNP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792601
Spacegroup name	P 21 21 21
Unit cell lengths	48.461, 55.078, 114.621
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.1959
Rwork	0.194
R-free	0.23250
RMSD bond length	0.010
RMSD bond angle	1.351
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.058	0.033	0.636
Rmeas	0.066	0.037	0.710
Rpim	0.030	0.017	0.309
Number of reflections	26341
<I/σ(I)>	11.5
Completeness [%]	99.6	95.4	100
Redundancy	4.8	4.3	4.8
CC(1/2)		0.997	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	297	0.2 M Potassium phosphate monobasic, 20%PEG3350