5VA7
Glucocorticoid Receptor DNA Binding Domain - IL11 AP-1 recognition element Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.143, 96.855, 104.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.793 - 2.153 |
R-factor | 0.2027 |
Rwork | 0.201 |
R-free | 0.22700 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
High resolution limit [Å] | 2.180 | 4.700 | 2.180 |
Rmerge | 0.085 | 0.064 | 0.575 |
Rmeas | 0.094 | 0.072 | 0.672 |
Rpim | 0.040 | 0.032 | 0.340 |
Total number of observations | 117427 | ||
Number of reflections | 21954 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.6 | 99.5 | 96.4 |
Redundancy | 5.3 | 4.9 | 3.5 |
CC(1/2) | 0.993 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Lithium Nitratie, 15 % PEG 3350, 1 % glycerol |