5VA4
TRIM-Cyclophilin A B-box 2 and coiled-coil chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-02-05 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | I 2 3 |
Unit cell lengths | 104.596, 104.596, 104.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.954 - 2.306 |
R-factor | 0.2364 |
Rwork | 0.231 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eiu |
RMSD bond length | 0.008 |
RMSD bond angle | 0.375 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.045 | 0.681 |
Number of reflections | 8544 | 1281 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 10.6 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 17% PEG 3350, 0.4 M sodium/potassium tartrate |