5V97
Structure of the H477R variant of rat cytosolic PEPCK in complex with GTP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.281, 118.983, 60.974 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 32.800 - 1.800 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qey |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.080 | 0.048 | 0.607 |
| Total number of observations | 303532 | ||
| Number of reflections | 57690 | ||
| <I/σ(I)> | 20 | ||
| Completeness [%] | 99.1 | 98.6 | 94.2 |
| Redundancy | 5.3 | 6.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 24 - 34% PEG 3350 and 100mM HEPES, at pH 7.4 |
