5V90
Crystal structure of ERp29 D-domain in complex with the P-domain of calreticulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.6307 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.573, 68.573, 167.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.340 - 3.255 |
| R-factor | 0.2413 |
| Rwork | 0.240 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qc7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 6735 | 368 |
| <I/σ(I)> | 19.1 | 6.9 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 9.7 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.0 M ammonium sulfate, 0.1 M HEPES pH 7.5 |
