5V8Y
Mutant Structures of Streptococcus Agalactiae GBS Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 147.081, 147.081, 72.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.667 - 1.950 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jyf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.649 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.099 | 0.822 |
| Rmeas | 0.108 | 0.964 |
| Rpim | 0.040 | 0.492 |
| Number of reflections | 52755 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 90.6 | 76.3 |
| Redundancy | 5.9 | 3.5 |
| CC(1/2) | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 20% PEG 3350, 0.2M MgCl2, 0.1 M Mes pH 6.0 |
