5V8Y

Mutant Structures of Streptococcus Agalactiae GBS Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.5418
Spacegroup name	P 4 21 2
Unit cell lengths	147.081, 147.081, 72.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.667 - 1.950
R-factor	0.1937
Rwork	0.192
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jyf
RMSD bond length	0.004
RMSD bond angle	0.649
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.11.1-2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.099	0.822
Rmeas	0.108	0.964
Rpim	0.040	0.492
Number of reflections	52755
<I/σ(I)>	11.6
Completeness [%]	90.6	76.3
Redundancy	5.9	3.5
CC(1/2)		0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	20% PEG 3350, 0.2M MgCl2, 0.1 M Mes pH 6.0