5V8K

Homodimeric reaction center of H. modesticaldum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0088
Spacegroup name	C 1 2 1
Unit cell lengths	131.358, 89.714, 111.637
Unit cell angles	90.00, 108.15, 90.00

Refinement procedure

Resolution	29.080 - 2.200
R-factor	0.16
Rwork	0.158
R-free	0.19100
RMSD bond length	0.012
RMSD bond angle	1.570
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (DEV_2450)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.280
High resolution limit [Å]	2.200	2.200
Rmerge		0.480
Number of reflections	61415
<I/σ(I)>	16.6
Completeness [%]	98.0	89
Redundancy	1.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	0.3 mM HbRC, 100 mM Tris-HCl (ph 8.2), 600 mM NH4Cl, 0.02 % b-DDM, up to 1% n-heptyl-b-D-glucopyranoside, up to 20 % PEG 500