5V8C
LytR-Csp2A-Psr enzyme from Actinomyces oris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.850, 69.070, 82.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.830 - 2.510 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.830 | 52.830 | 2.570 |
High resolution limit [Å] | 2.510 | 11.200 | 2.510 |
Rmerge | 0.051 | 0.045 | 1.014 |
Rmeas | 0.056 | 0.050 | 1.105 |
Number of reflections | 8332 | 112 | 572 |
<I/σ(I)> | 16.37 | 37.04 | 1.58 |
Completeness [%] | 98.9 | 94.1 | 93.5 |
Redundancy | 6.354 | 5.152 | 6.309 |
CC(1/2) | 0.998 | 0.997 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M sodium citrate pH 5.5, 25% PEG4000, 20% 2-propanol, 5 mM DTT, 200 mM NaCl, 5 mM MgCl2 |