5V8A
Crystal structure of Influenza A virus matrix protein M1 (NLS-88R, pH 7.3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-04-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.664, 33.341, 36.151 |
| Unit cell angles | 112.18, 100.38, 94.21 |
Refinement procedure
| Resolution | 21.926 - 3.000 |
| R-factor | 0.2751 |
| Rwork | 0.272 |
| R-free | 0.32130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ea3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.595 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.081 | 0.512 |
| Number of reflections | 2187 | 269 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 94.5 | 93.2 |
| Redundancy | 2.83 | 2.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | Protein solution: 10 mg/ml in K2HPO4/KH2PO4, 0.2M NaCl, 10bME, pH 7.2. Reservoir solution: 0.2M NaF, 20% PEG-3350, pH 7.3 |
