5V7Y

Prolyl 4-Hydroxylase Interacts with and Modifies Elongation Factor Tu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-04-19
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	50.238, 106.826, 81.056
Unit cell angles	90.00, 103.65, 90.00

Refinement procedure

Resolution	63.397 - 2.050
R-factor	0.1635
Rwork	0.161
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iax
RMSD bond length	0.012
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.400	63.400	2.110
High resolution limit [Å]	2.050	8.940	2.050
Rmerge	0.092	0.024	0.475
Rmeas	0.108	0.028	0.560
Rpim	0.057	0.014	0.294
Number of reflections	51889
<I/σ(I)>	13.3
Completeness [%]	99.5	98.1	99.9
Redundancy	3.6	3.6	3.6
CC(1/2)	0.996	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M imidazole pH 8.0, 5% PEG 4000, 15% MPD