5V7S

Crystal structure of Influenza A virus matrix protein M1 (NLS-88E, pH 6.2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	85.612, 133.260, 39.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.230 - 2.500
R-factor	0.2251
Rwork	0.220
R-free	0.31150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ea3
RMSD bond length	0.009
RMSD bond angle	1.165
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.230	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.077	0.449
Number of reflections	14742	1473
<I/σ(I)>	12.1	3.6
Completeness [%]	90.7	91.9
Redundancy	5.37	5.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	Protein solution: 8 mg/ml in 50mM K2HPO4/KH2PO4/H3PO4, 0.2M NaCl, 10mM bME, pH 3.2 Reservoir solution: 0.1M Tris-HCl, pH 8.2, 8% PEG-8K