5V7O

Crystal Structure of NosK from Streptomyces actuosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	75.290, 75.290, 110.121
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.053 - 2.300
R-factor	0.2232
Rwork	0.219
R-free	0.26270
RMSD bond length	0.007
RMSD bond angle	0.863
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.053	28.050	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.170	0.044	1.155
Number of reflections	16566
<I/σ(I)>	12.7
Completeness [%]	99.9	97.1	100
Redundancy	10.8	9	11.1
CC(1/2)	0.980	0.972	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.6 M (NH4)2SO4, 0.1 M NaCl, 0.1 M HEPES, pH 7.5