5V7L

PCNA mutant R61A/D63A Protein Defective in Gene Silencing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-20
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	123.181, 123.181, 123.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 3.201
R-factor	0.2017
Rwork	0.199
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.550
High resolution limit [Å]	3.200
Number of reflections	10563
<I/σ(I)>	24.9
Completeness [%]	100.0
Redundancy	22.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M sodium citrate, pH 5.57, 0.671M ammonium sulfate, 0.994M lithium sulfate