5V7K
PCNA mutant D41A/D42A Protein Defective in Gene Silencing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-13 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 122.323, 122.323, 122.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.830 - 3.050 |
| R-factor | 0.1863 |
| Rwork | 0.184 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.927 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | Force Field X |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 14.830 |
| High resolution limit [Å] | 3.050 |
| Number of reflections | 11773 |
| <I/σ(I)> | 16.1 |
| Completeness [%] | 99.1 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M sodium citrate, pH 5.43, 0.529 ammonium sulfate, 0.729M lithium sulfate |
