5V7K
PCNA mutant D41A/D42A Protein Defective in Gene Silencing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-09-13 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 3 |
Unit cell lengths | 122.323, 122.323, 122.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.830 - 3.050 |
R-factor | 0.1863 |
Rwork | 0.184 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.927 |
Data reduction software | d*TREK |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | Force Field X |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 14.830 |
High resolution limit [Å] | 3.050 |
Number of reflections | 11773 |
<I/σ(I)> | 16.1 |
Completeness [%] | 99.1 |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M sodium citrate, pH 5.43, 0.529 ammonium sulfate, 0.729M lithium sulfate |