5V7J

Crystal Structure at 3.7 A Resolution of Glycosylated HIV-1 Clade A BG505 SOSIP.664 Prefusion Env Trimer with Four Glycans (N197, N276, N362, and N462) removed in Complex with Neutralizing Antibodies 3H+109L and 35O22.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 63
Unit cell lengths	131.160, 131.160, 315.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.423 - 2.907
R-factor	0.3296
Rwork	0.316
R-free	0.33910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tvp
RMSD bond length	0.013
RMSD bond angle	1.632
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.423	3.000
High resolution limit [Å]	2.907	2.900
Rpim	0.131	0.432
Number of reflections	33547
<I/σ(I)>	3.82
Completeness [%]	51.3
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	4.95% isopropanol, 8.25% PEG 3350, 0.2M ammonium citrate, pH 4.5