5V7B
Crystal structure of Influenza A virus matrix protein M1 (NLS-88E)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-08 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.190, 96.170, 48.490 |
Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
Resolution | 27.580 - 2.500 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ea3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.910 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.072 | 0.379 |
Number of reflections | 12404 | 1227 |
<I/σ(I)> | 9.5 | 2.7 |
Completeness [%] | 98.7 | 99.3 |
Redundancy | 3.57 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 277 | 16.7 mg/ml protein in 25mM Hepes/NaOH, 0.2M NaCl, 2mM TCEP, pH 7.0 |