5V76

Structure of Haliangium ochraceum BMC-T HO-3341

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 3
Unit cell lengths	110.571, 110.571, 110.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.090 - 1.550
R-factor	0.1577
Rwork	0.157
R-free	0.17090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ht5
RMSD bond length	0.009
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev_2650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.103	1.797
Number of reflections	65376	9459
<I/σ(I)>	26.9	2.6
Completeness [%]	99.9	100
Redundancy	42.6	44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1M cacodylate pH 6.5 1.25M citrate