5V6T
The Plexin D1 intracellular region in complex with GIPC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 99.777, 99.777, 531.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.033 - 3.189 |
| R-factor | 0.1819 |
| Rwork | 0.179 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v6r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.041 | 0.361 |
| Number of reflections | 27629 | |
| <I/σ(I)> | 17.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris pH 5.5 and 2 M (NH4)2SO4 |
