5V6L
Crystal Structure of Rabbit Anti-HIV-1 gp120 V3 Fab 10A37 in complex with V3 peptide JR-FL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.100000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.234, 173.904, 70.912 |
Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
Resolution | 44.000 - 2.549 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jo1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.049 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.440 |
High resolution limit [Å] | 2.549 | 2.298 |
Number of reflections | 33285 | 6977 |
<I/σ(I)> | 15.6 | 3.8 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 5.1 | 5.1 |
CC(1/2) | 0.996 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 18% polyethylene glycol 4000, 8.5% Isopropanol, 0.1 M HEPES pH 7.5, 15% glycerol |