5V6I
Glycan binding protein Y3 from mushroom Coprinus comatus possesses anti-leukemic activity - Pt derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.102, 55.237, 41.879 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 41.300 - 1.700 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 1.760 |
| High resolution limit [Å] | 1.699 | 1.700 |
| Rmerge | 0.093 | 0.443 |
| Number of reflections | 20217 | |
| <I/σ(I)> | 14.57 | |
| Completeness [%] | 99.0 | 87 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 16% PEG4000, 10% V/V GLYCEROL, 0.1 M CHES, PH 9.5 |
