5V6H
Crystal structure of Myosin VI in complex with GH2 domain of GIPC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.848, 53.240, 122.764 |
Unit cell angles | 90.00, 108.23, 90.00 |
Refinement procedure
Resolution | 42.929 - 3.601 |
R-factor | 0.2194 |
Rwork | 0.213 |
R-free | 0.27770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.603 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Rpim | 0.079 | 0.270 |
Number of reflections | 9763 | |
<I/σ(I)> | 11 | |
Completeness [%] | 98.7 | 95.4 |
Redundancy | 4.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.5 and 10% (w/v) PEG20000 |