5V6F
Crystal Structure of the Second beta-Prism Domain of RbmC from V. cholerae bound to Mannotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-26 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.882, 38.581, 50.954 |
| Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
| Resolution | 16.112 - 1.500 |
| R-factor | 0.1664 |
| Rwork | 0.164 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xez |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.663 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 16.112 | 16.112 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.033 | 0.481 | |
| Rmeas | 0.097 | 0.038 | 0.647 |
| Rpim | 0.056 | 0.018 | 0.427 |
| Total number of observations | 48096 | 2729 | 4411 |
| Number of reflections | 18634 | 628 | 2442 |
| <I/σ(I)> | 7.7 | 29.2 | 1.5 |
| Completeness [%] | 95.1 | 95.7 | 86.1 |
| Redundancy | 2.6 | 4.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate, 2 M sodium sulfate, 2 mM mannotriose |
