5V6C

Crystal Structure of the Second beta-Prism Domain of RbmC from V. cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-03
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	90.636, 92.664, 100.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.530 - 2.200
R-factor	0.1762
Rwork	0.173
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xez
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	CrysalisPro
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.530	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.347	1.372
Rmeas	0.375	1.489
Rpim	0.140	0.573
Total number of observations	154289	13314
Number of reflections	21750	1994
<I/σ(I)>	7.5	2.1
Completeness [%]	99.7	100
Redundancy	7.1	6.7
CC(1/2)	0.978	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1 M sodium acetate, 2 M sodium sulfate