5V6B
Crystal structure of GIPC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.396, 77.625, 80.346 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 40.173 - 1.900 |
| R-factor | 0.1403 |
| Rwork | 0.136 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v6t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.900 | |
| Rpim | 0.034 | 0.319 |
| Number of reflections | 44113 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.2 M Li2SO4, 0.1 M HEPES pH 7.5 and 25% (w/v) PEG3350 |
